海洋微微型真核生物分子多样性的研究方法     

汪岷  江雪娇 《中国海洋大学学报(自然科学版)》2009,39(4)

微微型真核生物在海洋中广泛存在,是初级生产力的重要贡献者,也是微食物环的重要组成部分.由于微微型真核生物形体微小且不同种类缺乏明显形态学特点,故而难以利用电镜等传统方法精确研究其系统发生关系和种类组成.运用分子生物学技术直接对微微型真核生物种类组成进行研究已成为微微型真核生物多样性研究的重要方法.本文概述了目前世界范围内的微微型真核生物分子多样性研究进展以及我国在该领域中的研究状况.综述了微微型真核生物分子多样性研究中常用的靶标基因,包括18S rDNA和ITS序列等真核生物保守基因及psbA和rbcL等功能基因;常用的分子生物学方法,如克隆文库构建、DGGE/TGGE、PCR-RFLP/T-RFLP、FISH、宏基因组等;以及目前研究方法中存在的一些问题.  相似文献   

三沙湾西北部滨海湿地潮间带生物生态研究   总被引：1，自引：0，他引：1  

黄雅琴  李荣冠  郑成兴  郑凤武  王建军  林俊辉  李淑珠  江锦祥 《台湾海峡》2009,28(2):279-292

本文报道三沙湾西北部滨海湿地潮间带生物109种,其中多毛类、软体动物和甲壳动物占总种数的89.90%,三者构成潮间带生物主要类群.断面间比较,Sch1断面的种数最少（42种）,Sch3断面的种数最多（73种）.种类季节变化,以春季种数（83种）大于秋季（59种）.三沙湾西北部滨海湿地潮间带生物平均生物量12.45g/m^2,平均栖息密度为368个/m^2.生物量以软体动物居第一位（7.34g/m^2）,甲壳动物居第二位（2.55g/m^2）;栖息密度以软体动物居第一位（196个/m^2）,多毛类居第二位（154个/m^2）.数量垂直分布,生物量以中潮区（15.20g/m^2）大于高潮区（12.37g/m^2）大于低潮区（9.78g/m^2）;栖息密度以低潮区（563个/m^2）大于中潮区（483个/m^2）大于高潮区（56个/m^2）.数量季节变化,生物量以春季（16.69g/m^2）大于秋季（8.21g/m^2）;栖息密度以春季（677个/m2）大于秋季（58个/m^2）.三沙湾西北部滨海湿地潮间带生物按断面和所处的位置可分为3个群落：Sch1泥沙滩群落,高潮区：黑口滨螺-粗糙滨螺带;中潮区：中蚓虫-鸭嘴蛤-秀丽长方蟹带;低潮区：似蛰虫-焦河篮蛤-鸭嘴蛤带.Sch2泥沙滩群落,高潮区：黑口滨螺-粗糙滨螺带;中潮区：才女虫-鸭嘴蛤-淡水泥蟹带;低潮区：似蛰虫-侧底理蛤-鸭嘴蛤带.Sch3泥沙滩群落,高潮区：黑口滨螺-粗糙滨螺带;中潮区：才女虫-鸭嘴蛤-短拟沼螺-弧边招潮带;低潮区：不倒翁虫-侧底理蛤-齿腕拟盲蟹带.结果分析表明：春季Sch1和Sch2群落不稳定,秋季Sch1、Sch2群落和Sch3群落相对稳定,总体显示,三沙湾西北部滨海湿地潮间带生物群落发生扰动,初步分析认为与优势种鸭嘴蛤和优势种才女虫季节演替有关.  相似文献   

湛江海域文昌鱼(Branchiostoma)线粒体Cyt b基因的全序列分析     

郭昱嵩  王中铎  刘楚吾  刘筠 《广东海洋大学学报》2008,28(1):6-10

测定了邻近湛江的茂名海区和硇洲岛海域文昌鱼各2个样本线粒体Cytb基因全序列,以尾索动物亚门的2种海鞘(Ciona spp.)和脊椎动物亚门的七鳃鳗(Petromyzon marinus)作为外群,利用分子系统学方法与GenBank收录的14个文昌鱼(Branchiostoma)的Cytb基因全序列进行了比较,UPGMA法构建了系统发生树。全序列分析表明:主产于大西洋(批针文昌鱼B.lanceolatum和佛罗里达文昌鱼B.floridae)、厦门文昌鱼(B.belcheri belcheri Gray)和湛江文昌鱼线粒体Cytb基因全序列长度为1143bp,终止密码子同为UAG;主产于青岛文昌鱼(B.belcheri tsingtaunese)和日本文昌鱼为1141bp,以T结尾,转录过程中构成UAA终止密码子。系统分析表明:16个文昌鱼样品体现出太平洋和大西洋、太平洋南北明显分化。确认采自茂名和硇洲岛的文昌鱼为厦门文昌鱼(B.belcheribelcheriGray);支持将白氏文昌鱼青岛亚种(B.belcheri tsingtaunese)提升为种,命名为日本文昌鱼(B.japonicus)。  相似文献   

青岛、威海水域夏冬季表层沉积物细菌多样性的初步研究   总被引：4，自引：0，他引：4  

肖慧  张艳  张喆  朱琳  游偲  唐学玺 《中国海洋大学学报(自然科学版)》2009,39(4)

以青岛、威海水域夏冬两季表层沉积物中的基因组DNA为模板,扩增沉积物样品中细菌的16S rDNA 片段,并构建其相应文库.进行16S rDNA序列分析,并与数据库中的序列进行比对.分析结果表明:2个站位沉积物样品细菌分别属于11个主要细菌门类,包括:变形菌门(Proteobacteria)、浮霉菌门(Plancomycene)、芽单胞菌门(Gemmatimonadetes)、酸杆菌门(Acidobacteria)、硝化螺菌门(Nitrospirae)、疣微菌门(Verrucomicrobial)、拟杆菌门(Bacteroidetes)、绿屈挠菌门(Chloroflexi)、厚壁菌门(Firmicutes)、高G+C含量革兰氏阳性菌放线菌门(Actinobacteria)和低G+C含量革兰氏阳性菌梭菌门(Clostridiales).其中变形菌门是主要类群,且γ-变形菌纲(γ-Proteobacteria)占优势.与QD2站位相比,QD1站位沉积物细菌多样性随季节变化不大.  相似文献   

凡纳滨对虾4个选育群体遗传多样性的SSR分析   总被引：6，自引：0，他引：6  

谢丽  陈国良  叶富良  栗志民 《广东海洋大学学报》2009,29(4):5-9

采用9对微卫星引物分析了凡纳滨对虾4个选育群体(Molokai、OI、SIS和Kona Bay)的遗传多样性。结果表明:9对微卫星引物共检测到32个等位基因,每个位点等位基因数2～7个,平均3.555 6个,平均有效等位基因数2.472 7;各位点的观测杂合度(Ho)0.026 0～0.623 4,期望杂合度(He)0.263 1～0.785 5;各群体平均观测杂合度从小到大依次为SIS(0.383 3)相似文献   

An analysis of sponge diversity and distribution at three taxonomic levels in the Thousand Islands/Jakarta Bay reef complex, West-Java, Indonesia   总被引：1，自引：0，他引：1  

Nicole J. de Voogd  & Daniel F. R. Cleary 《Marine Ecology》2008,29(2):205-215

Very few coral reefs are located close enough to large cities to study the influence of large urban populations on reef assemblages. An exception is the Thousand Islands reef complex to the north of Jakarta, the capital city of Indonesia, and one of the largest conurbations in the world. Here we present data from a detailed survey where sponge assemblages were assessed at 30 patch reefs associated with islands in three coastal zones along an in-to-offshore gradient. Sponge assemblages are described at three taxonomic levels of detail (species, genus and family level). We recorded a total of 118 sponge species, 64 genera and 36 families, Aaptos suberitoides (Brønsted, 1934), Clathria (Thalysias) reinwardti (Vosmaer, 1880), Petrosia (Petrosia) nigricans (Lindgren, 1897) and Xestospongia testudinaria (Lamarck, 1813) were the most common species overall. There was a pronounced difference in composition among zones with the most distinct difference between the inshore zone and the other two zones. The inshore environment was characterised by very high turbidity and low live coral cover; the dominant substrate consisted of algal turf and sand. Environmental conditions improved and sponge diversity increased further offshore, although most areas appeared to have been affected by some form of disturbance. Ordinations were also largely congruent, at species, generic and family levels of taxonomic resolution. This indicates that variation in composition at higher taxonomic levels is a good indicator of variation at lower taxonomic levels, at least when there is a pronounced environmental gradient.  相似文献   

青岛裙带菜孢子体野生种群遗传多样性的AFLP分析   总被引：2，自引：1，他引：1  

张东  隋正红  易恒  仝舟  包振民 《中国海洋大学学报(自然科学版)》2009,39(4)

采用优化的基因组总DNA提取方法,按照经典的AFLP分析路线,对30株青岛近海野生裙带菜(Undaria pinnatifida)孢子体种群内遗传多样性进行了AFLP分析.利用1对引物扩增出38条清晰可重复的带,其中32条呈现多态性,多态位点比例为84.21%,根据引物扩增的指纹图谱,计算出的种群内部平均观测等位基因数(Na)为1.8421、平均有效等位基因数(Ne)为1.375 4、平均基因多样性指数,即Nei's基因多样性指数(H)为0.235 1,Shannon's 多样性信息指数(I)为0.368 4.该种群内遗传距离的分布范围从0.026 7～0.804 4,结果显示,野生种群内个体间各项遗传指数水平均较高,种群内遗传变异丰富.聚类分析表明,在遗传距离为0.21的阈值处,30个个体划分为2大类群,推测青岛野生裙带菜进化上在遗传距离为0.21时发生第一次分化.  相似文献   

Genetic diversity and population structure of the golden cuttlefish,Sepia esculenta (Cephalopoda: Sepiidae) indicated by microsatellite DNA variations   总被引：1，自引：0，他引：1  

Xiaodong Zheng  Minoru Ikeda  Lingfeng Kong  Xiangzhi Lin  Qi Li  Nobuhiko Taniguchi 《Marine Ecology》2009,30(4):448-454

The golden cuttlefish, Sepia esculenta Hoyle, 1885 (Cephalopoda: Sepiidae) is a valuable and important fishery resource for China, Japan and Korea. This fishery has experienced severe population decline largely due to overexploitation in past decades. To provide guidelines for fisheries management, we estimated genetic diversity and population structure across four locations along the coast of Japan and one location in China (a total 281 individuals) using nine microsatellite DNA loci. Sepia esculenta showed high genetic variability with mean allele richness ranging from 15.8 to 21.4, and mean heterozygosity from 0.80 to 0.90. Weak but significant genetic differentiation was present and the F_ST value was 0.020 across all five populations. The useful information obtained in this study will offer insights into how to fine‐tune conservation and fishery management measures for this species and resource in the future.  相似文献   

Spatial patterns of biodiversity in the Black Sea: An assessment using benthic polychaetes     

Victor Surugiu  Nikolai Revkov  Valentina Todorova  Nafsika Papageorgiou  Vasilis Valavanis  Christos Arvanitidis 《Estuarine, Coastal and Shelf Science》2010

The current study broadens the biodiversity information available for the Black Sea and neighbouring regions and improves our knowledge about the polychaete biogeographic patterns to be discerned in them. There appears to be a well-defined zoogeocline from the Marmara Sea and Bosphorus Strait to the inner parts of the region (Azov Sea), depicted both as a multivariate pattern and in terms of species (or taxa) numbers. The emergent multivariate pattern complies, to a certain extent, with Jakubova’s (1935) views: three main sectors can be defined in the basin: (a) Prebosphoric, (b) the Black Sea and, (c) the Azov Sea, whereas the Bosphorus Strait and Marmara Sea show less faunal affinities with the afore-mentioned sectors. Patterns derived both from the cosmopolitan and Atlanto-Mediterranean species closely follow the one coming from the polychaete species and genera inventories. As a general trend, species numbers decrease along with the decrease in salinity towards the inner parts of the region. The trend is homologous to that seen in the benthic invertebrate inventories of all the major European semi-enclosed regional seas. Salinity and food availability appear to be the dominant abiotic factors correlated, though weakly, with the various patterns deriving from the taxonomic/zoogeographic categories. With the exception of the Anatolia, polychaete inventories from all sectors appear to be random samples of the total inventory of the region, in terms of taxonomic distinctness values. Therefore, these sectoral inventories can be used for future biodiversity/environmental impact assessment studies. A massive invasion of Mediterranean species after the opening of the Black Sea, in the lower Quaternary period, appears to be the likely biogeographic mechanism through which the old Sarmatic fauna was almost completely replaced by species of marine origin.  相似文献   

荣成靖海湾海参养殖池塘初级生产力季节变化特征   总被引：1，自引：0，他引：1  

任贻超  王芳  董双林  刘峰 《中国海洋大学学报(自然科学版)》2010,40(3)

2007年5月~2007年11月调查了山东荣成靖海湾海参养殖池塘水体中浮游植物种类组成、生物量、叶绿素含量变化以及底泥沉积物中叶绿素和脱镁叶绿素含量的变化.结果表明,海参养殖池塘水体中浮游植物共有7门,44属,62种,主要以硅藻为主.水体中浮游植物平均生物量1号池塘为17.52×10~4 /L;2号池塘为18.30×10~4 /L.水体叶绿素含量变化范围:1号池塘为1.38～5.00 μg/L;2号池塘为0.98～5.45 μg/L.池塘沉积物中叶绿素含量变化范围:1号池塘为0.66～1.67 μg/g,2号池塘为0.56～1.34 μg/g.池塘沉积物中脱镁叶绿素含量变化范围:1号池塘为2.97～5.63 μg/g;2号池塘为2.90～6.36 μg/g.1号池塘和2号池塘的浮游植物群落结构及叶绿素含量没有显著性差异(P>0.05).叶绿素,脱镁叶绿素变化趋势较为一致.海参夏眠期间,沉积物中叶绿素及脱镁叶绿素含量最高,为海参结束夏眠进入摄食提供食物储备.研究结果表明,靖海湾海参养殖池塘浮游植物以硅藻为主,养殖池塘水体浮游植物生物量较低,多样性指数较高,增加浮游植物沉降速率,增加养殖系统初级生产力可为海参提供更多食物.  相似文献